METHOD OF IDENTIFYING POLYMORPHISM R287Q IN 8 EXON GENE CYTOSOLIC EPOXIDE HYDROLASE IN HUMAN BEINGS Russian patent published in 2009 - IPC C12Q1/68 

FIELD: chemistry, biochemistry.

SUBSTANCE: invention relates to the region of molecular biology and can be used in medicine during diagnostics of predisposition to multi-factorial diseases in man. Method of detecting polymorphism R287Q in 8 exon gene cytosolic epoxide hydrolase (EPHX2) with the method PCR-RLFP using direct primer 5'-CTG-TGG-CTC-TTG-GTT-TGA-TC-3' and reverse primer 5'-TTC-ATG-TCC-ATA-GCT-AGG-ATC-3', at the 3'-end to which is introduced a restriction site for endonuclease BamHI, used later for hydrolysis of PCR-of the product is offered. In this case obtaining during fractionating in the agarose gel two fragments of DNA of the size 143 and 21 p.o are verified as homozygous genotype of the wild type 287RR, three fragments size 164, 143 and 21 p.o - as the heterozygous genotype 287RQ, and one of the fragments size 164 p.o - as homozygous mutant genotype 287QQ. Offered is a method of genotyping and by achieved with minimal material costs in the laboratory, with standard equipment for carrying out polymeric chain reactions.

EFFECT: developing an effective and economic method of detecting polymorphism R287Q in 8 exon gene cytosolic epoxide hydrolase.

2 dwg, 1 ex