FIELD: oil and gas industry.
SUBSTANCE: invention relates to receiving of exo-polysaccharides used in the capacity of thickeners during the operation of oil fields, particularly to method of receiving exo-polysaccharides of alginate type. Method provides cultivation of Azotobacter vinelandii "ИБ-1" on culture medium by Fedorov, containing mixture of microelements and molasses in amount 60.0 g/l in fermenter at conditions of aeration; 0.5 of air volumes per 1 medium volume per minute and mixing 300 revolutions per minute at temperature 25°C during 48 hours. Release of exo-polysaccharides by means of sedimentation by isopropyl alcohol in amount 70% by volume.
EFFECT: increasing of exo-polysaccharides yield.
1 tbl, 7 ex
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Authors
Dates
2009-06-20—Published
2007-03-26—Filed