FIELD: biotechnology.
SUBSTANCE: thrombin derivative is described that includes chain A and chain B where chain B has an aminoacid sequence whose aminoacids of the series active center in position 205 and histidine in position 43 in the aminoacid sequence of chain B of thrombin are substituted and where: the specified thrombin derivative decomposes thrombin substrate to the extent of 10% or less when interacting with thrombin substrate in 50 mM Tris-HCl (pH 7.4) containing 0.1 M NaCl at the temperature of 37°C for 3 hours and the specified thrombin derivative retains the ability of getting combined with C-terminal hirudin peptide immobilised in gel.
EFFECT: pharmaceutical formula is disclosed that contains the thrombin derivative described.
54 cl, 48 dwg, 2 tbl, 31 ex
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