FIELD: medicine.
SUBSTANCE: DNA-aptamer specifically and high-affinity get bound with thrombin and is described by general formula: dRGTGACGTANGGTTGGTGTGGTTGGGGCGTCACR (1) where N is any nucleotide of G, A, T, C line or can be absent; R is a chemical radical of 5'-O-methyl-, 5'O-alkyl, 5'-Oallyl; 3'-O-methyl, 3'O-alkil, 3'-O-allyl or can be absent; d is deoxyribose (oligonucleotide contains deoxyribose in all positions).
EFFECT: invention allows producing DNA-aptamer which high-affinity get bound with thrombin, inhibits fibrin-forming activity of purified thrombin, inhibits thrombin activity in human blood plasma, with prolonging activated partial thromboplasmin time, prothrombin and thrombin time, as well as inhibits thrombin stimulated thrombocyte aggregation mediated by thrombin banding with PAR thrombocyte receptors.
2 cl, 13 dwg, 5 ex
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