METHOD FOR MAKING DIAGNOSTIC ALLERGEN OF PLANT POLLEN Russian patent published in 2009 - IPC A61K36/36 

FIELD: medicine.

SUBSTANCE: invention concerns pharmacology and medicine. It involves water-salt extraction of Canadian goldenrod pollen pre-defatted by ethyl ester and dried. Extraction of fat-free and dried Canadian goldenrod pollen is carried out with Evans-Cook liquid during three days with daily agitation at temperature 15-26°C three times for 30-40 minutes every 1-1.5 hours. In intervals between agitations the material is kept at temperature 4-8°C. After extraction, supernatant fluid is poured out that is followed with centrifugation at 5000-6000 rpm within 30-40 minutes and filtration through a paper filter. Then sterilisation filtration, stabilisation of the mother liquor of allergen within 1-3 months and cultivation thereof after finished diagnostic allergen is made.

EFFECT: invention allows making a preparation not causing a sensitisation in animals in a diagnostic dose, possessing specific activity in reaction of indirect degranulation of rat's mast cells.

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