METHOD FOR OBTAINING TICK ALLERGEN FOR DIAGNOSTIC SAMPLING Russian patent published in 2005 - IPC

FIELD: pharmacology, medicine.

SUBSTANCE: as initial raw material one should apply tick culture of domestic dust. Extracting should be conducted for 45 h with 0.125 M ammonium hydrocarbonate solution, supernatant should be poured and centrifuged for 45 min, extract should be filtered followed by dialysis for 52 h at 6±2 C, twice per 24 h against 0.002 M ammonium hydrocarbonate solution and once for 4 h against distilled water. Dialyzed extract should be centrifuged for 1 h, filtered through paper filter to fulfill freeze drying up to residual moisture being 4%, not more. Dry residue should be dissolved in 0.1 M phosphate buffer at pH being 7.5. Then one should add 1%-formaldehyde solution and perform sterilizing filtration. Formalinized allergoid should be kept for 32 d at 32 C. Then comes dialysis at 6±2 C against 0.1 M phosphate buffer solution at pH being 7.5. The innovation enables to develop the allergen of decreased allergenic activity.

EFFECT: higher efficiency.

1 ex