METHOD FOR IDENTIFICATION OF Bacillus anthracis WITH DIFFERENTIATION OF STRAINS BY PRODUCTION OF CAPSULE, PROTECTIVE ANTIGEN AND ANTIGENS OF S-LAYER Russian patent published in 2009 - IPC C12Q1/04 C12R1/07 G01N33/559 

Abstract RU 2376385 C1

FIELD: medicine.

SUBSTANCE: virulent anthrax bacillus strains are grown on dense nutrient medium. Antigens of S-layer are diffused into nutrient agar and are detected with appropriate serums by creation of immunoprecipitation lines. Capsule is identified visually by morphology of colonies and with the help of light microscopy of smears colored by Rebiger. At the same time method uses non-plasmid strain B. anthracis 81/1TR and toxigenic strain B. anthracis STI.

EFFECT: developed method is an affordable method, considerably simplifies generally accepted scheme for identification of anthrax causative agent and reduces time required for analysis performance.

12 dwg, 1 tbl, 8 ex

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RU 2 376 385 C1

Authors

Barkova Irina Anatol'Evna

Barkov Anatolij Makarovich

Alekseev Vladimir Valer'Evich

Lipnitskij Anatolij Vasil'Evich

Dates

2009-12-20Published

2008-07-07Filed