FIELD: medicine.
SUBSTANCE: virulent anthrax bacillus strains are grown on dense nutrient medium. Antigens of S-layer are diffused into nutrient agar and are detected with appropriate serums by creation of immunoprecipitation lines. Capsule is identified visually by morphology of colonies and with the help of light microscopy of smears colored by Rebiger. At the same time method uses non-plasmid strain B. anthracis 81/1TR and toxigenic strain B. anthracis STI.
EFFECT: developed method is an affordable method, considerably simplifies generally accepted scheme for identification of anthrax causative agent and reduces time required for analysis performance.
12 dwg, 1 tbl, 8 ex
Authors
Dates
2009-12-20—Published
2008-07-07—Filed