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2 492 241

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IPC

C12P21/00(2006-01-01)

A61K39/02(2006-01-01)

C12N1/21(2006-01-01)

C12R1/07(2006-01-01)

(21) (22)

Application

2012129190/10, 2012-07-10

(24)

Start date

2012-07-10

(22)

Actual filing date

2012-07-10

(45)

Published

2013-09-10

(72)

Inventor

Mikshis Natal'Ja IvanovnaGoncharova Anastasija Jur'EvnaPopova Polina Jur'EvnaKudrjavtseva Ol'Ga MikhajlovnaPopov Jurij AlekseevichKutyrev Vladimir Viktorovich

(73)

Holder

Federal'Noe Kazennoe Uchrezhdenie Zdravookhranenija Nauchno-Issledovatel'Skij Protivochumnyj Institut Federal'Noj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija Cheloveka

METHOD OF PRODUCTION OF PROTECTIVE ANTIGEN AND PROTEIN OF S-LAYER EA1OF ASPOROGENOUS RECOMBINANT STRAIN IN anthracis 55 ΔTPA-1Spo Russian patent published in 2013 - IPC C12P21/00 A61K39/02 C12N1/21 C12R1/07

Abstract RU 2492241 C1

FIELD: biotechnology.

SUBSTANCE: method includes cultivation of previously prepared culture of the recombinant strain B. anthracis 55ΔTPA-1Spo^-. The cell mass is separated using the filtration module with a membrane having a pore diameter of 0.2 mcm. Protein EA1 is extracted from the washed cell mass using a buffer with 1% sodium dodecyl sulfate, and purified by diafiltration using membrane filters and two-stage ion-exchange chromatography on hydroxyapatite. The protective antigen is isolated from the culture filtrate and purified by successive steps of concentration and diafiltration.

EFFECT: use of the invention enables to obtain in one processing chain the highly purified antigens of anthrax microbe - protective antigen and protein EA1 needed to create chemical vaccines.

3 dwg, 5 ex

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RU 2 492 241 C1

Authors

Mikshis Natal'Ja Ivanovna

Goncharova Anastasija Jur'Evna

Popova Polina Jur'Evna

Kudrjavtseva Ol'Ga Mikhajlovna

Popov Jurij Alekseevich

Kutyrev Vladimir Viktorovich

Dates

2013-09-10—Published

2012-07-10—Filed