METHOD OF QUANTITATIVE DETERMINATION OF PENETRATION OF MANGANESE SUCCINATE TETRAHYDRATE INTO INCUBATIVE CHICKEN EGGS Russian patent published in 2010 - IPC A01K43/00 

FIELD: agriculture.

SUBSTANCE: incubative eggs of the control and test batches are treated with Succ-Mn solution by means of vacuum suction. After treatment eggs are incubated in the incubator for 24 hours. After that eggs from each batch are taken for further studies and for that purpose samples of protein and yolk are put into a thermostat at a temperature of +60°C and dried until the weight is constant. The biomaterial obtained in this way is studied by means of atomic absorption spectrometry for microelements of manganese, copper, zinc, iron as well as for sodium by means of flame-photometric method. On day 19, several embryonated eggs are taken from each batch. Embryos with yolk sac and them without it are weighed whereupon each embryo without yolk sac are homogenised. Amount of micromoles Succ-Mn passed through the eggshell is calculated by the formula: where: X is amount of Succ-Mn tetrahydrate penetrated into the egg, mmole; 288.94 is molecular weight of Succ-Mn (C₈H₁₀O₈Mn), T is manganese concentration in the test sample, mmole/egg; K is manganese concentration in the control sample, mmole/egg; 54.94 is manganese atomic mass.

EFFECT: structure and physical and chemical properties of manganese succinate tetrahydrate are determined as well as quantitative account of its penetration when performing one-time preincubative treatment of eggs with solutions with different concentrations.

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