FIELD: medicine.
SUBSTANCE: method involves growing tissue culture Rauwolfia serpentina Benth (Rauwolfia serpentina) K-27 strain in a nutrient medium of given composition in the presence of melaphen (melamine salt of bis (hydroxymethyl) phosphinic acid) of 10-17 passages at a concentration of melaphen 1·10-3 g/l. After that received tissue culture Rauwolfia serpentina Benth is transplanted to the nutrient medium of the same composition but containing very small concentrations of melaphen 1·10-10-1·10-15 g/l, mainly at 1·10-13 g/l and is grown in this medium for a passage not more than 50 days.
EFFECT: increase in the accumulation of alkaloids.
3 cl, 5 dwg, 2 tbl
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