METHOD FOR OBTAINING OF BIOLOGICALLY ACTIVE SUBSTANCES IN CONIUM MACULATUM L CELL CULTURE Russian patent published in 2017 - IPC C12N5/04 A61P37/04 

FIELD: biotechnology.

SUBSTANCE: invention is a method for biologically active substances preparation in Conium maculatum L cell culture, comprising Conium maculatum callus culture culturing on a nutrient medium MC in the presence of 6-benzylaminopurine for 30 days, biomass harvesting and selection of biologically active substances; the difference is that the cultivation is carried out at 26±1°C, 70% humidity, in the dark, 6-benzylaminopurine is introduced into the nutrient medium at a concentration of 1 to 0.1 mg/l together with 2.4-dichlorophenoxyacetic acid at a concentration in the range of 1 to 0.5 mg/l or together with α-naphthylacetic acid at a concentration in the range of 3 to 0.5 mg/l, and the biologically active substances are isolated by acidic and alkaline chloroform extraction.

EFFECT: invention allows to obtain the required amount of callus culture biomass, containing BAV-furanocoumarins and acyclic triterpenoids.

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