FIELD: biotechnology.
SUBSTANCE: invention relates to the field of virology and biotechnology, in particular, to a strain of hybrid cultured animal cells Mus musculus 1F1. Hybrid clone of 1F1 cells was produced by fusion of mouse myeloma cells of the Sp2/0-Ag-14 line with splenocytes of mice of the Balb/c line, immunised intramuscularly with the recombinant protein N of the SARS-CoV-2 virus. Hybridoma 1F1 produces a monoclonal antibody of the IgG1 class, specifically interacting with the recombinant nucleocapsid protein N and the inactivated SARS-CoV-2 virus. The resulting antibody can be used in diagnostic studies to identify an antigen and specific antiviral antibodies.
EFFECT: invention expands the range of means for producing monoclonal antibodies against the nucleocapsid protein N of the SARS-CoV-2 virus.
1 cl, 1 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF HYBRID ANIMAL CELLS MUS MUSCULUS 2E1B5 - PRODUCER OF A MONOCLONAL ANTIBODY AGAINST THE RECEPROT-BINDING DOMAIN OF PROTEIN S OF THE SARS-CoV-2 VIRUS | 2021 |
|
RU2771288C1 |
| THE STRAIN OF HYBRID CULTURED HOMO SAPIENS/Mus MUSCULUS CELLS Hu-C6D7-RBD IS A PRODUCER OF HUMAN MONOCLONAL ANTIBODIES SPECIFIC TO THE RBD DOMAIN S OF THE SARS-CoV-2 VIRUS PROTEIN | 2022 |
|
RU2788359C1 |
| MONOCLONAL ANTIBODY TO RDB FRAGMENT IN COMPOSITION OF SARS-COV-2 S PROTEIN | 2020 |
|
RU2744274C1 |
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|
RU2686630C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus musculus L - PRODUCER OF MONOCLONAL ANTIBODIES FOR EXPOSING VP40 PROTEIN OF MARBURG VIRUS, (Popp STRAIN) (VERSIONS), MONOCLONAL ANTIBODY PRODUCED BY SAID STRAIN (VERSIONS) AND SET FOR "SANDWICH" FORMAT IMMUNOENZYMOMETRIC SYSTEM FOR EXPOSING VP40 MATRIX PROTEIN OF MARBURG VIRUS (Popp STRAIN) | 2008 |
|
RU2395575C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus musculus L - PRODUCER OF MONOCLONAL ANTIBODIES FOR EXPOSING VP40 PROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) (VERSIONS), MONOCLONAL ANTIBODY PRODUCED BY SAID STRAIN (VERSIONS) AND SET FOR "SANDWICH" FORMAT IMMUNOENZYMOMETRIC TEST SYSTEM FOR EXPOSING VP40 PROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) | 2008 |
|
RU2395577C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus museums L - PRODUCER OF MONOCLONAL ANTIBODIES FOR EXPOSING NUCLEOPROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) (VERSIONS), MONOCLONAL ANTIBODY PRODUCED BY SAID STRAIN (VERSIONS) AND SET FOR "SANDWICH" FORMAT IMMUNOENZYMOMETRIC TEST SYSTEM FOR EXPOSING NUCLEOPROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) | 2008 |
|
RU2395576C1 |
| STRAIN OF HYBRID ANIMAL CELLS MUS MUSCULUS L5F10-PRODUCER OF MONOCLONAL ANTIBODIES USED FOR DETECTING PP65 PROTEIN OF HUMAN CYTOMEGALOVIRUS | 2008 |
|
RU2393219C1 |
| STRAIN OF HYBRID ANIMAL CELLS MUS MUSCULUS L3F9 - PRODUCER OF MONOCLONAL ANTIBODIES SUITABLE FOR USE IN "SANDWITCH" IMMUNOENZYMOMETRIC SYSTEM FORMAT FOR DETECTING VP35 PROTEIN OF MARBURG VIRUS AND 3F9 MONOCLONAL ANTIBODIES PRODUCED BY SAID STRAIN OF HYBRID CELLS | 2008 |
|
RU2393220C1 |
| STRAIN OF HYBRID CELLS OF ANIMAL MUS MUSCULUS L, 9E2, USED IN PREPARING MONOCLONAL ANTIBODY TO WEST NILE VIRUS PROTEIN E OF STRAIN WNV/LEIV-VIG99-27889 | 2004 |
|
RU2265658C2 |
