DEAMIDATED BETA INTERFERON Russian patent published in 2010 - IPC C07K14/565 A61K38/19 A61P31/12 A61P25/00 

FIELD: medicine.

SUBSTANCE: invention relates to protein analogues of interferon-β, in which asparagine in position 25, in accordance with numeration of amino acids in native interferon-β, is deamidated, and cysteine in position 17, in accordance with numeration of amino acids in native interferon-β, is deleted or substituted with neutral amino acid, which demonstrate higher levels of biological activity of native human interferon-β and do not require HA for protein stabilisation. Deamidated product id suitable for wide-scale production for introduction into HA-containing or HA-non-containing therapeutic medications for treatment of diseases including disseminates sclerosis. Also claimed is method of peptide mapping by means of endoproteinase-C, which gives chromatographic profile of proteins with use of enzymatic breakdown with further RP-HPLC, is suitable for quality control as ID and/or quantitative test of deamidated products.

EFFECT: improvement of biological properties.

21 cl, 16 dwg, 6 tbl