FIELD: medicine.
SUBSTANCE: invention relates to field of medicine, namely to diagnostic methods. Homogenate of placenta of pregnant woman who had herpes-virus infection is analysed by ELISA method for activity of granzyme B, titre of antibodies in pregnant woman's peripheral blood, and simultaneously number of syncyotrophoblast nuclei in apoptosis is calculated. If antibody titre is 1:3200 and granzyme B content is 283.14±18.00 pg/ml, percentage of nuclei in apoptosis state is 1.5±0.09%; if antibody titre is 1:6400 and granzyme B content in placenta homogenate is 712.84±23.00 pg/ml content of nuclei in apoptosis state is 2.0±0.12%; if titre of antibodies to herpes virus is 12800 and content of granzyme B in placenta homogenate is 1007.53±31.50 pg/ml (control - 251.10±31,00 pg/ml) percentage of nuclei in state of apoptosis increases to 3.5±0.08% (control - 1.0±0.009%).
EFFECT: method ensures high sensitivity of detecting nuclei in state of apoptosis in syncyotrophoblast at the background of increased content of granzyme B in placenta homogenate.
