FIELD: food industry.
SUBSTANCE: biomass production envisages cultivation of at least one lactic acid bacteria strain producing vitamin K2 under conditions of resting cells. One performs at least primary cultivation of bacteria under conditions of respiration sustention for production of the primary culture or pre-culture in a medium containing at least one compound containing a haem nucleus, the end concentration being at least nearly 0.5 mcg/ml. A fat-containing milk cultural medium is inoculated with live bacterial cells in an amount of approximately 108 CFU/ml - 1011 CFU/ml. The inoculated medium is fermented during approximately 4 hours - 48 hours at a temperature of approximately 4°C - 50°C. The lactic acid bacteria are chosen from the species Lactococcus lactis, Leuconostoc lactis, Leuconostoc pseudomesenteroides, Leuconostoc mesenteroides, Leuconostoc dextranicum, Enterococcus faecium and Propionibacterium sp.
EFFECT: one produces biomass with a level of vitamin K2 producing from approximately 30 mcg to approximately 75 mcg and more of vitamin K2 per 100 g of the fermented cultivation medium.
16 cl, 6 dwg, 5 tbl
