FIELD: chemistry.
SUBSTANCE: method involves oxidation of reducing substances with copper (II) compounds in an alkaline medium while heating on a boiling water bath for 10 minutes, bringing the volume of the cooled solution to a defined value, determining optical density at wavelength 670 nm, making an adjustment to partial oxidation of non-reducing carbohydrates, which is subtracted from the optical density reading, and estimating concentration of reducing substances from a calibration curve.
EFFECT: high accuracy and reproducibility of analysis.
5 ex, 6 tbl, 4 dwg
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Authors
Dates
2012-07-27—Published
2010-04-06—Filed