FIELD: pharmacology.
SUBSTANCE: method for common sugars determination in a thin sorbent layer is characterized by treatment of a portion of the investigated drug with water in the presence of concentrated hydrochloric acid at boiling in a water bath with subsequent chromatography in an n-butanol-acetic acid-water (4:1:2) eluent, detection by a solution containing 0.86 g of sulfanilamide and 0.83 g of o-phthalic acid in 50 ml of 95% ethanol and drying at a temperature of 103±2°C for 3-5 minutes before chromatographic areas appear; after development of the chromatographic areas, plates are scanned using a flatbed scanner, and the resulting images are treated with a known computer program "Sorbfil Videodensitometer", common sugar areas are identified by their characteristic the value of Rf and their quantitative content in the sample is calculates.
EFFECT: increased accuracy of determination.
3 ex, 5 dwg, 3 tbl
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