PROTEIN CAUSEING CELL AUTOAGGLUTINATION PROPERTY OF PLAGUE MICROBES, AND METHOD FOR PRODUCING IT Russian patent published in 2013 - IPC C07K14/195 C12P21/00 C12R1/00 

FIELD: medicine, pharmaceutics.

SUBSTANCE: protein causing an Yersinia pestis cell autoagglutination property and located on a surface of the Yersinia pestis cell is recovered, and the presence thereof is correlated with an ability of bacteria to the autoagglutinaiton growth in liquid nutrient media and with increased hydrophobic nature of the cells. Molecular weight of the protein makes 17.485 kDa, the protein content is 80%, and the neutral carbohydrate content is 6%. A method for producing the protein involves growing the strain of Y.pestis KM 1279 on 1.5% agar LB; the grown culture is washed off with cold buffered saline and dried with acetone. The cells are washed off and deposited by centrifugation in the cold; the cell deposition is suspended in NaOH (pH 9.0), kept in agitation at 26°C for two hours, and the cells are separated by centrifugation (8000 rpm). The supernatant is filtered through the membrane; the filtrate is added with neutralised ammonium sulphate to 25% saturation, kept to form a deposition to be separated by centrifugation and made free from ammonium sulphate by dialysis. The dialysed solution is added with HCl to pH 4.6, incubated to form a deposition, centrifuged; the deposition is dissolved in 5 mM NaOH. The protein is deposited by acetone, kept in the cold, centrifuged; and the deposition is lyophilised.

EFFECT: protein is used for studying its protective properties, for creating on its basis vaccination preparations and assessing its involvement in realisation of the virulent properties of the plague agent.

2 cl, 1 dwg, 2 tbl, 4 ex