METHOD FOR QUANTITATIVE ANALYSIS OF SPECIFIC IMMUNOGLOBULINS G TO CONJUGATE OF FORMALDEHYDE-SERUM HUMAN ALBUMIL IN BLOOD SERUM Russian patent published in 2013 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: when implementing the method for quantitative analysis of specific immunoglobulins G to a conjugate of formaldehyde - human serum albumin in blood serum, test and reference nitro-cellulose substrate are prepared by placing them in various microplate wells with said test substrate prepared by sequential sorption of human serum albumin and formaldehyde thereon at room temperature for 8 hours, while the reference substrate is prepared by sorption of human serum albumin thereon. It is followed by incubation of the test and reference substrates with the analysed serum sample for 30 minutes. After the substrates are washed in a phosphate buffer, the substrates are added with monoclonal antibodies with Fc-fragments of human immunoglobulin G conjugated with horseradish peroxidase; the substrates are washed in the phosphate buffer once more, and an immunochemical complex of specific immunoglobulin G antibodies are coloured by adding chromogene and developed by adding a stop reagent, The worked out substrates are removed from the microplate wells, and optical density is recorded in the test and reference samples; they are matched with optical density of the reference samples of the known concentration of immunoglobulin G, and a difference of the content of immunoglobulin G in the test and reference samples shows a number of patient's blood serum immunoglobulin G specific to the test substance provided optical density in the test sample exceeding that of the reference sample not less than 1.5 times.

EFFECT: method enables higher accuracy and reliability of determining specific immunoglobulins G in blood serum.

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