METHOD OF ASSESSMENT OF DISORDER OF CELLULAR IMMUNITY IN EXPOSED TO PHENOL Russian patent published in 2014 - IPC G01N33/53 

FIELD: biotechnology.

SUBSTANCE: for evaluation of disorders of cellular immunity when exposed to phenol the blood sampling and its analysis is carried out using experimental and control samples with preliminary introduction to the test sample of toxicant - phenol in a concentration corresponding to its regional background level. As a criterion factors in the method the index of specific sensitivity of cells is used for cytokine tumour necrosis factor TNF-α and the index of specific sensitivity of cells to cytokine interleukin-10 IL-10, which are defined as follows: the experimental and control blood samples are placed into the vial with a sterile nutrient medium DMEM with heparin and gentamicin in a volume ratio of 1:4, respectively (in a preferred embodiment, the contents in the sterile nutrient medium DMEM of heparin is 2.5 U/ml and gentamicin - 100 mcg|ml), incubation of these samples is carried out at 37°C during 24 hours, followed by centrifugation for 3 minutes at 1000 rev/min, and in the selected supernatant of the test sample the level of IPC_IL-10 phenol-induced production of cytokine IL-10 is determined and the level of IPC_TNF-α phenol-induced production of cytokine - tumour necrosis factor TNF-α, and in the selected supernatant of the control sample the level of spontaneous cytokine production is determined, namely the SPC_IL-10 level of spontaneous production of cytokine IL-10 and SPC_TNF-α level of spontaneous production of the cytokine TNF-α, then ISS_TNF-α index of specific sensitivity of cells for cytokine TNF-α is calculated, and the ISS_IL-10 index of specific sensitivity of cells for cytokine IL-10 is calculated using the following formulas: ISS_TNF-α=(IPC_TNF-α- SPC_TNF-α): SPC_TNF-α, ISS_IL-10 =(IPC_IL-10- SPC_IL-10): SPC_IL-10, where IPC_IL-10 is the level of phenol-induced production of cytokine IL-10; IPC_TNF-α is the level of phenol-induced production of cytokine TNF-α; SPC_IL-10 is the level of spontaneous production of cytokine IL-10; SPC_TNF-α is the level of spontaneous production of the cytokine TNF-α; and when meeting the simultaneous requirement of ISS_{TNF-α of} over 10 and ISS_IL-10 of over 2 the disorder of cellular immunity of the organism is determined under the influence of phenol.

EFFECT: increase in reliability of making assessment of impact of phenol on disorder of cellular immunity of the human body.

2 cl, 8 tbl