METHOD FOR SEPARATION AND ORGANOTYPIC PRESERVATION OF ALLOGENIC LIMB TRANSPLANT Russian patent published in 2013 - IPC A61F9/00 A61K31/721 A61K35/44 A61P27/02 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely ophthalmology, and may be used in separation and organotypic preservation of an allogenic limb transplant. A method involves early surgical separation of the limb transplant from a whole corpse donor eyeball with eliminated perforation after donor's death. The transplant is preserved within 28 days in a medium containing insulin, dexamethasone, blood serum, penicillin, streptomycin, dextran 40000, HEPES M-solution, 7.5% bocarbonate, 40% glucose, L-glutamine, penicillin, streptomycin, amphotericin B and Hanks salt medium 199 under certain proportion of the components. The preservation is performed at temperature 37°C with the medium changed every 3 days. The same medium, but serum-free is used for washing. The method provides preserving transplant viability with proliferating progenitor cells that leads to achieving stable, long-term epithelisation, stroma defect recovery, prevention of corneal conjunstivisation.

EFFECT: prepared transplant may be used for surgical management of the patients with limb cell insufficiency syndrome of various aetiologies.

2 cl, 1 ex, 9 dwg