METHOD OF PREPARING BIOLOGICAL MATERIAL FOR ISOLATION OF Coxiella burnetii DNA Russian patent published in 2013 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: invention lies in the following: 4-5 ml of blood from cubital vein are sampled into standard test tubes for polymerase chain reaction with sputtering on walls of disodium salt of ethylenediaminetetraacetic acid, which is kept for three hours to precipitate erythrocytes, after which 700-800 mcl of plasma are sampled and dilutes with 0.11% phosphate buffer, containing 20 mM of disodium salt of ethylenediaminetetraacetic acid with ratio 1:1, after which it is centrifuged for 10 minutes at 700 g with obtaining upper layers of plasma, which contain platelets, to isolate which 800 mcl of supernatant plasma are connected with ACD-buffer in ratio 8:1 with recentrigugation for 10 minutes at 12000 g with obtaining deposit of platelets, suitable for detection of Coxiella burnetii DNA by method of polymerase chain reaction with hybridisation- fluorescence detection in real time mode.

EFFECT: invention makes it possible to increase quality of genome of Coxiella burnetii DNA.

19 dwg, 6 ex