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(19)

(11)

2 525 059

(13)

(51)

IPC

C12Q1/68(2006-01-01)

C12N15/11(2006-01-01)

(21) (22)

Application

2012130988/10, 2012-07-19

(24)

Start date

2012-07-19

(22)

Actual filing date

2012-07-19

(45)

Published

2014-08-10

(72)

Inventor

Frejlikhman Ol'Ga AleksandrovnaTokarevich Nikolaj KonstantinovichPanferova Julija Aleksandrovna

(73)

Holder

Federal'Noe Bjudzhetnoe Uchrezhdenie Nauki Nauchno-Issledovatel'Skij Institut Ehpidemiologii I Mikrobiologii Im. Pastera" Nii Ehpidemiologii I Mikrobiologii Imeni Pastera)

KIT FOR DETECTING Q FEVER AGENT IN BIOLOGICAL MATERIAL BY REAL TIME POLYMERASE CHAIN REACTION (RT PCR) Russian patent published in 2014 - IPC C12Q1/68 C12N15/11

Abstract RU 2525059 C2

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and concerns a kit for detecting Q fever by RT PCR. The characterised kit contains synthetic oligonucleotides limiting a fragment of groEL gene: GroEL F 5' CTTCTACTGTTATGACGCCTTCTTTGC 3'; GroEL R 5' CGCAAGTAGGCACCATTTCTGC 3', and a fluorescent probe: GroEL Probe 5' FAM-CACTTTCTCCATCGCTTCCGCAATAATA-TAMRA 3'.

EFFECT: invention can be used in medicine, veterinary science, clinical laboratory diagnostics for the recovery of the bacteria Coxiella burnetii DNA in test samples, as well as for solving scientific problems aiming at examining the given microorganism.

2 tbl, 2 ex

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RU 2 525 059 C2

Authors

Frejlikhman Ol'Ga Aleksandrovna

Tokarevich Nikolaj Konstantinovich

Panferova Julija Aleksandrovna

Dates

2014-08-10—Published

2012-07-19—Filed