FIELD: medicine.
SUBSTANCE: invention refers to biotechnology and concerns a kit for detecting Q fever by RT PCR. The characterised kit contains synthetic oligonucleotides limiting a fragment of groEL gene: GroEL F 5' CTTCTACTGTTATGACGCCTTCTTTGC 3'; GroEL R 5' CGCAAGTAGGCACCATTTCTGC 3', and a fluorescent probe: GroEL Probe 5' FAM-CACTTTCTCCATCGCTTCCGCAATAATA-TAMRA 3'.
EFFECT: invention can be used in medicine, veterinary science, clinical laboratory diagnostics for the recovery of the bacteria Coxiella burnetii DNA in test samples, as well as for solving scientific problems aiming at examining the given microorganism.
2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| KIT FOR DETECTING LEPTOSPIROSIS IN BIOLOGICAL MATERIAL THROUGH REAL-TIME POLYMERASE CHAIN REACTION (RT-PCR) METHOD | 2019 |
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RU2627607C1 |
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| METHOD FOR DETECTING THE PRESENCE OF MUTATIONS LEADING TO MYCOPLASMA GENITALIUM RESISTANCE TO MACROLIDE AND FLUOROQUINOLONE ANTIBIOTICS | 2019 |
|
RU2725477C1 |
| METHOD FOR HEPATITIS B VIRUS DNA IDENTIFICATION IN BIOLOGICAL MATERIAL WITH LOW VIRAL LOAD BASED ON TWO-STAGE PCR | 2016 |
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RU2753309C2 |
