METHOD OF CONTROLLING PROCESSES OF OBTAINING AND DRYING ENZYME PREPARATIONS Russian patent published in 2013 - IPC C12M1/02 C12M1/36 F26B5/06 

FIELD: chemistry.

SUBSTANCE: enzyme preparations are obtained using a fermenter with a heating jacket for submerged culturing of microorganisms of the enzyme preparations with continuous aeration with compressed air and mechanical agitation at culturing temperature of 30…32°C on the entire volume of the fermenter; the culture liquid obtained in the fermenter is cultured to remove the residue and the filtrate of the culture liquid with moisture content of 92…95% is fed into a vacuum-sublimation drier, in which the desublimator used is a two-section evaporator of a vapour compression refrigerating machine, the working and spare section of which alternately operate in condensation and regeneration modes, respectively; wherein "hot" water is obtained by heating thereof in the condenser of the refrigeration machine using condensation heat of the coolant to temperature of 68…73°C, one part of which is fed into the heating jacket of the fermenter and the other is first fed for thawing the evaporator section operating in regeneration mode, and then the water cooled to temperature of 5…7°C is then removed from the evaporator section into a storage tank together with the liquid formed from the ice cover thawed on the surface of the cooling element in an amount of moisture evaporated from the enzyme preparation, and further, in closed cycle mode, fed in two streams, one of which is mixed with waste water after the fermenter before the condenser, and the second with "hot" water before the fermenter, wherein excess water from the recirculation loop is removed through the storage tank, followed by measurement of the culturing temperature in the fermenter, the flow rate and temperature of the water at the inlet of the heating jacket of the fermenter, the flow rate and moisture content of the filtrate of the culture liquid, residual pressure in the working volume of the vacuum-sublimation drier, the moisture content of the dried enzyme preparation, the flow rate and temperature of water vapour removed from the vacuum-sublimation drier into the working section of the evaporator, the boiling point of the coolant in the working section of the evaporator, the temperature of uncondensed vapour at the outlet of the working section of the evaporator and the level of water in the storage container; mass and heat flow of the mixture of cooled and "hot" water into the heating jacket of the fermenter is established by changing the ratio of flow rates thereof with adjustment of the culturing temperature in the fermenter; the measured values of moisture content and flow rate of the filtrate of the culture liquid are used to determine the power of the drive of the compressor of the refrigeration machine and the required residual pressure in the working volume of the sublimation drier by action on the power of the controlled drive of the vacuum pump with adjustment of the residual pressure on the final moisture content of the enzyme preparation; the flow rate and temperature of water vapour removed from the vacuum-sublimation drier into the working section of the evaporator, the temperature of uncondensed vapour at the outlet of the working section of the evaporator and the boiling point of the coolant in the working section of the evaporator are used to determine the current value of the coefficient of heat transfer from the water vapour to the coolant on the cooled surface of the working section of the evaporator, and upon achieving minimum permissible value of the coefficient of heat transfer, power of the drive of the compressor of the refrigeration machine is increased first, and the working section of the evaporator is then switched from condensation mode to regeneration mode while simultaneously switching to condensation mode the section operating in regeneration mode.

EFFECT: improved quality of enzyme preparations by increasing accuracy and reliability of controlling the process parameters, high energy efficiency and environmental safety of production processes and vacuum-sublimation drying.

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