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2 481 400

(13)

(51)

IPC

C12P19/30(2006-01-01)

C12Q1/68(2006-01-01)

C12N1/20(2006-01-01)

C12R1/225(2006-01-01)

(21) (22)

Application

2011152710/10, 2011-12-22

(24)

Start date

2011-12-22

(22)

Actual filing date

2011-12-22

(45)

Published

2013-05-10

(72)

Inventor

Semenikhin Vladimir IvanovichJurik Sofija Antonovna

(73)

Holder

Gosudarstvennoe Nauchnoe Uchrezhdenie Institut Ehksperimental'Noj Veterinarii Sibiri I Dal'Nego Vostoka Rossijskoj Akademii Sel'Skokhozjajstvennykh Nauk Iehvsidv Rossel'Khozakademii)

SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD TO DETECT Lactobacillus delbrueckii subspecies bulgaricus IN STARTER CULTURES USED IN PRODUCTION OF CULTURED MILK FOODS Russian patent published in 2013 - IPC C12P19/30 C12Q1/68 C12N1/20 C12R1/225

Abstract RU 2481400 C1

FIELD: biotechnologies.

SUBSTANCE: invention relates to oligonucleotide primers and the method of their use for detection of Lactobacillus delbrueckii subspecies bulgaricus in starter cultures. Proposed synthetic oligonucleotide primers have nucleotide sequences: Lbul4F 5'-GGCCAGCCAGATCGCCAGC-3' and Lbul5R 5'-GACCAGGTCGCTGTCCGGC-3'. The proposed method includes performance of PCR. In case a DNA ferment is detected with size of 409 base pairs. a conclusion is made on availability of Lactobacillus delbrueckii subspecies bulgaricus in the investigated biomaterial.

EFFECT: inventions may be used in milk processing industry for detection and identification of strains and cultures Lactobacillus delbrueckii subspecies bulgaricus in starter cultures used in production of cultured milk foods.

2 cl, 1 tbl, 3 ex

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RU 2 481 400 C1

Authors

Semenikhin Vladimir Ivanovich

Jurik Sofija Antonovna

Dates

2013-05-10—Published

2011-12-22—Filed