FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology and concerns a method for identifying lactic acid bacilli. The presented method is based on a combination and a polymorphism of toxin-antitoxin RelBE and MazEF gene superfamilies and characterised by the fact that identifying is ensured by genome DNA amplification with using a set of oligonucleotides of certain structure; PCR products are analysed in agarose gel, while a size of the produced fragment is determined by means of a DNA marker. The analysed strain is referred to a specific group, species or strain in case of producing fragments when using certain oligonucleotides.
EFFECT: presented invention can be used for identifying the lactic acid bacilli strains in human microbiota, food stuff, as well as for detecting the analysed strain in clinical samples, or molecular targeting in commercial preparations.
3 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF SPECIES AND STRAIN IDENTIFICATION OF BIFIDOBACTERIA OF PHYLOTYPE Bifidobacterium longum | 2011 |
|
RU2527069C2 |
| METHOD OF SPECIES IDENTIFICATION OF L.casei/paracasei, L.fermentum, L.plantarum AND L.rhamnosus | 2012 |
|
RU2508406C2 |
| LACTOBACILLUS BREVIS AND LACTOBACILLUS RHAMNOSUS STRAINS WITH ESTABLISHED GENOMIC SEQUENCY SYNTHESIZING GLUTATHION AND INTRACELLULAR ANTIOXIDANTS COMPLEX | 2015 |
|
RU2617946C1 |
| Lactobacillus rhamnosus strain, POSSESSING ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY AGAINST PATHOGENIC AND OPPORTUNISTIC VAGINAL MICROFLORA | 2013 |
|
RU2570622C2 |
| METHOD OF GENOTYPING STRAINS Mycobacterium tuberculosis | 2013 |
|
RU2547598C1 |
| METHOD FOR PREPARING L-THREONINE USING MICROORGANISM BELONGING TO GENUS ESCHERICHIA WHEREIN mazEF OPERON IS INACTIVATED | 2005 |
|
RU2313573C2 |
| STRAINS OF Lactobacillus plantarum AND Lactobacillus brevis SYNTHESISING GAMMA-AMINOBUTYRIC ACID | 2014 |
|
RU2575625C1 |
| LACTOBACILLUS FERMENTUM U-21 STRAIN, WHICH PRODUCES COMPLEX OF BIOLOGICALLY ACTIVE SUBSTANCES WHICH NEUTRALIZE SUPEROXIDE ANION INDUCED BY CHEMICAL AGENTS | 2018 |
|
RU2705250C2 |
| RECOVERING, IDENTIFYING AND DESCRIBING STRAINS WITH PROBIOTIC ACTIVITY RECOVERED FROM FAECES OF EXCLUSIVELY BREAST-FED CHILDREN | 2010 |
|
RU2551315C2 |
| METHOD FOR CELL METABOLISM CONTROL | 2019 |
|
RU2731897C1 |
