METHOD TO DETECT β-LACTAMASE-PRODUCING OXIDASE-POSITIVE GRAM-NEGATIVE DIPLOCOCCI, SUSPICIOUS FOR APPURTENANCE TO MORAXELLA (BRANCHAMELLA) CATARRHALIS Russian patent published in 2013 - IPC C12Q1/04 C12R1/01 

FIELD: biotechnologies.

SUBSTANCE: method according to the invention discloses the possibility to detect β-lactamase-producing oxidase-positive gram-negative diplococci, suspicious for appurtenance to Moraxella (Branchamella) catarrhalis and provides for inoculation of a clinical material on blood-serum agar in sectors with simultaneous application of discs with penicillin 10 AU. The inoculation is incubated under regular atmospheric conditions for 18-24 hours, and in 36-48 hours from the moment of inoculation they assess availability or absence of a zone of growth delay for those microorganisms that are present in the clinical material. If there is no zone of microorganisms growth suppression around discs, they detect availability in the clinical material β-lactamase-producing oxidase-positive gram-negative diplococci. Appurtenance of detected diplococci to Moraxella (Branchamella) catarrhalis is identified by biochemical criteria.

EFFECT: simplified extraction of sought-for microorganisms from a contaminated clinical material.

1 tbl