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2 787 047

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IPC

C12N15/64(2006-01-01)

C12N15/66(2006-01-01)

C12Q1/04(2006-01-01)

(21) (22)

Application

2021135815, 2021-12-06

(24)

Start date

2021-12-06

(22)

Actual filing date

2021-12-06

(45)

Published

2022-12-28

(72)

Inventor

Kandinov Ilya DenisovichAntonova Olga VladimirovnaGryadunov Dmitrij AleksandrovichDementeva Ekaterina IgorevnaShaskolskij Boris LeonidovichKubanov Aleksej AlekseevichSolomka Viktoriya SergeevnaDeryabin Dmitrij Gennadevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Nauki Institut Molekulyarnoj Biologii Im. V.A. Engelgardta Rossijskoj Akademii Nauk Ran)

PLASMID VECTOR pESB FOR ASSESSING THE RESISTANCE OF NEISSERIA GONORRHOEAE TO BETA-LACTAM ANTIBIOTICS AND METHOD FOR CONSTRUCTION THEREOF Russian patent published in 2022 - IPC C12N15/64 C12N15/66 C12Q1/04

Abstract RU 2787047 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, microbiology, and medicine, and constitutes a constructed plasmid vector containing a gene encoding an extended-spectrum β-lactamase capable of hydrolysing penicillins and cephalosporins, and intended for replication in cells of Escherichia coli and Neisseria gonorrhoeae. The plasmid contains a plasmid DNA pbla_TEM-135 of N. gonorrhoeae wherein a fragment of the bla gene is cut and substituted with a similar fragment of the bla gene with the GGT→AGT mutation leading to the substitution of glycine with a series in codon 238 (Gly238Ser), allowing for the expression of extended-spectrum β-lactamase TEM-20 capable of hydrolysing penicillins and cephalosporins. Invention also discloses a method for constructing a plasmid vector.

EFFECT: possibility of obtaining a plasmid vector for assessing the resistance of N. gonorrhoeae to β-lactam antibiotics, including the products of choice for treating the gonococcal infection — III generation cephalosporins.

4 cl, 2 dwg, 1 tbl, 3 ex

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RU 2 787 047 C1

Authors

Kandinov Ilya Denisovich

Antonova Olga Vladimirovna

Gryadunov Dmitrij Aleksandrovich

Dementeva Ekaterina Igorevna

Shaskolskij Boris Leonidovich

Kubanov Aleksej Alekseevich

Solomka Viktoriya Sergeevna

Deryabin Dmitrij Gennadevich

Dates

2022-12-28—Published

2021-12-06—Filed