FIELD: medicine.
SUBSTANCE: method under the invention involves determining an anthrax lethal factor (LF) by PCR-associated immunodetection. The LF protein is adsorbed on microplates or microstrips with using a pre-adsorbed specific monoclonal antibody (MCAB). Further, the immobilised LF protein is bound by the biotylated MCAB recognising another epitope of the LF protein. Thereafter, a complex of the LF protein and the biotylated antibody is detected using a noncovalent conjugate of DNA fragments with neutravidin serving as a matrix for PCR amplification with real-time fluorescence signal detection. Fluorescence levels are recorded. Changing the fluorescence levels in the respective plate wells or strip tubes as compared to the reference, the presence of the lethal factor in the analysed samples is stated.
EFFECT: method for determining the anthrax lethal factor is characterised by high sensitivity and specificity, allowing diagnosing the lethal factor protein accumulation at the early stages of anthrax infection, beginning an antibiotic therapy of an infected patient in proper time.
5 dwg, 5 ex
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