METHOD FOR IMMUNOFLUORESCENT DETECTION OF PROTECTIVE ANTIGEN OF ANTHRAX AGENT Russian patent published in 2013 - IPC G01N33/53 G01N33/533 G01N33/543 G01N33/563 

FIELD: medicine.

SUBSTANCE: what is developed is a method for immunofluorescent high-sensitive detection of a protective antigen (PA) of an anthrax agent on the basis of monoclonal antibodies specific to various epitopes of the protective antigen one of which specific to the PA domain III is immobilised on a solid phase and serves for binding the PA contained in the sampled, while the second biotin-conjugated antibody specific to the domain IV detects the protective antigen due to the reaction with a tetravalent neutravidin molecule conjugated with phycoerythrin used for real-time fluorescent signal detection. The PA presence in the samples is shown by the varying fluorescence level as compared to the reference.

EFFECT: method is characterised by high sensitivity and enables early diagnosis of anthrax thereby preventing developing toxic pathology and a risk of fatal outcome and providing favourable outcome of the disease.

2 dwg, 1 tbl, 4 ex