METHOD FOR PREPARING COMPLEX OF BIOLOGICALLY ACTIVE SUBSTANCES OF COD LIVER Russian patent published in 2013 - IPC A61K35/60 A61K38/02 

FIELD: medicine.

SUBSTANCE: invention refers to medicine and veterinary science. What is presented is a method for preparing biologically active substances of cod (Gadidae) liver. For the purpose of preparing the biologically active substances, cod liver is homogenised; cut meat is processed with an extractant that is a mixture of vegetable oil and water in ratio of raw material: extractant 1:0.2-2. The processing is carried out at room temperature for 10 min to 1 h with mixing in a mixing machine at 50-200 rpm. Next, the ballast deposition is separated; macromolecular proteins are precipitated by treatment in an organic solvent. The solvent is specified in: 95% ethanol, acetone, acetonitrile, isopropanol, in the volumetric proportions of extractant-solvent 1:1-5. The treatment in the organic solvent is conducted at temperature -5 to +10°C. Thereafter, the suspension is settled at room temperature for 5-6 hours. The precipitation is separated by filtration to prepare an end product by drying the water residue.

EFFECT: stabilising the biologically active organic preparation, recovering the cell juice as much as possible, higher yield of the biologically active substances.

4 cl, 4 ex