METHOD FOR OBTAINING OF PEPTIDE IMMUNITY STIMULATOR (VERSIONS) AND BAA BASED THEREON Russian patent published in 2017 - IPC A61K38/17 A61P37/00 

FIELD: pharmacology.

SUBSTANCE: raw materials are defrost, ground, extracted with 3% acetic acid solution, the supernatant is obtained and separated, the final product is isolated. The raw material is extracted for 35-48 hours with stirring for 15 minutes every 1.5 hours, then basified with sodium bicarbonate solution to pH of 8.0-8.5, 0.3% chitosan solution in 3% acetic acid is added, the resulting mass is centrifuged on a centrifuge at 5000 rpm for 20 minutes, ammonium sulfate is added to the supernatant to 30% of the solution concentration, kept with stirring for two hours, centrifuged again at 5000 rpm for 20 minutes, the supernatant is diafiltered at the checkout counter hydrochloric membrane with a separation limit of 1000 Da four times with distilled water addition to the resulting concentrate with a concentrate: water ratio of 1:2 to obtain a solution with a final substance of 1000 to 15000 Da. Also biologically active additive is proposed.

EFFECT: increased biological activity by increasing the content of peptides with an immunostimulating effect.

7 cl, 2 tbl, 6 ex