FIELD: process engineering.
SUBSTANCE: set of invention relates to production of highly-enriched platelet concentrate from whole blood by means of centrifuge. Centrifuge comprises control unit with or without feedback and drive unit connected therewith, rotor equipped with at least two receiving plug-in containers communicated via fluid channel. Centrifuge every means is connected with receiving containers. Note here that said means are connected with said containers for initiation of blood components transfer there between. At least one separation layer detector is arranged between said containers and connected to aforesaid control unit. Note here that said means can be activated by motor with gearing connected with appropriate container and to aforesaid control unit. Closed system for sterile blood treatment comprises containers communicated with centrifuge. Every said container is composed of a tube accommodating piston reciprocating therein. Every said tube is engaged via piston push rod engaged in its turn with aforesaid motor and gearing coupled with appropriate container. Method of extraction of highly-enriched platelet from whole blood comprises fitting at least two tubes communicated via hose in aforesaid container. Note here that first tube contains whole blood while second tube contains, if required, a biocompatible material. Then, centrifuge is cut in and accelerated to first rpm to perform separation unless first separated high-density red blood cell concentrate and blood plasma enriched with platelet. Then, centrifuge is decelerated to first extrusion rpm. Then, said motor with gearing connected with first tube and piston is started to extrude said blood plasma enriched with platelet via hose into second tube unless aforesaid detector detects separation layer. Then, rpm is increased to second threshold to perform, second separation unless high-density platelet concentrate and blood plasma lean in platelet are formed. Then, centrifuge rpm is decrease to second magnitude to start said motor with gearing connected with second tune and piston so that blood plasma lean in platelet is extruded via hose back, in aforesaid first tube. Now, centrifuge and motor with gearing are shut down to withdraw the tubes.
EFFECT: simplified process.
16 cl, 5 dwg
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Authors
Dates
2013-11-10—Published
2010-09-07—Filed