FIELD: biotechnologies.
SUBSTANCE: production method of keratinase provides for directed adaptation of strain Penicillium citrinum PC-54-91"ВИЛАР" by three-time subcultivation on Czapek agar medium containing 2% of hair keratin as a carbon source. Cultivation under deep conditions on Czapek medium containing 10% of hair keratin and 0.5% of saccharose during 6 days. Separation of biomass from culture fluid with further extraction of target product by two-stage chromatographic cleaning involving gel filtration on TSK-Gel TOYOPEARL HW-40 and affine chromatography on protein A to sepharose CL-4B with further lyophilisation.
EFFECT: invention allows increasing ferment yield, obtaining keratinase preparation decomposing the hair α-keratin, and improving cleaning degree of ferment preparation.
2 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| ASPERGILLUS CLAVATUS STRAIN - PRODUCER OF PROTEOLITIC ENZYMES WITH KERATINOLYTIC ACTIVITY | 2021 |
|
RU2774366C1 |
| BACTERIAL STRAIN BACILLUS LICHENIFORMIS IS KERATINASE PRODUCER | 2023 |
|
RU2812484C1 |
| STRAIN OF BACTERIUM BACILLUS LICHENIFORMIS AS PRODUCER OF KERATINASE | 2000 |
|
RU2177994C2 |
| METHOD FOR PRODUCTION OF PROTHEOLYTIC REAGENT FOR STRUCTURAL PROTEIN HYDROLYSIS | 2003 |
|
RU2244741C1 |
| FUNGUS STRAIN PENICILLIUM ESTINOGENUM A KOMATSU ET S ABE EX G SM AS PRODUCER OF ENDONUCLEASE P AND METHOD FOR ITS PREPARING | 2002 |
|
RU2220198C1 |
| STRAIN OF STREPTOMYCES ORNATUS - A PRODUCER OF KERATINASE | 1993 |
|
RU2034924C1 |
| METHOD OF PRODUCING TARTRATE OF EPOXYAGROCLAVINE-1 AND QUINOCITRININES | 2008 |
|
RU2386692C1 |
| METHOD OF PRODUCING COMPACTIN | 2013 |
|
RU2585234C2 |
| 0 |
|
SU1565888A1 | |
| 0 |
|
SU201249A1 |
