METHOD OF PRODUCING TARTRATE OF EPOXYAGROCLAVINE-1 AND QUINOCITRININES Russian patent published in 2010 - IPC C12N1/14 C12P17/00 C12R1/80 

FIELD: chemistry; biochemistry.

SUBSTANCE: invention pertains to bioengineering. Penicillium citrinum VKM F-4043 D fungus is cultured in a mineral medium of the following composition (g/l): sucrose - 50.0-100, urea - 2.14-4.28, KH₂PO₄ -1.00-2.00, MgSO₄·7H₂O - 0.30-0.60, ZnSO₄·7H₂O -0.0044-0.0088 and distilled water - up to 1l. After separation of the biomass, epoxyagroclavine-1 is separated from the alkaline extract of the culture fluid through extraction with chloroform at acidic and alkaline pH values with subsequent dehydration and concentration. The obtained alkaline extract is dissolved in acetone and while stirring, a solution of tartaric acid in methanol is gradually added. Quinocitrinines are separated from the acidic extract of the culture fluid through column chromatography (with silica gel, ratio of the height to the diametre of the column equal to 1:1.8-2.0), with chloroform first and then with a mixture of chloroform and methanol in ratio 9:1.

EFFECT: method increases content of epoxyagroclavine-1 in the culture fluid, simplification of methods of purifying epoxyagroclavine-1 and quinocitrinines, reduced cost of the medium, longer period of storing epoxyagroclavine-1.

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