FLUORESCENCE DETECTION SYSTEM AND METHOD Russian patent published in 2014 - IPC G01N21/64 

FIELD: physics.

SUBSTANCE: fluorescence detection system has an exciting radiation source and a radiation processing device (18, 20), having a line forming element (20) and a beam shaping element (18), a focusing device, a device for collecting fluorescent or phosphorescent radiation, a detector (28), a substrate (16) for holding a sample (14) and a means of scanning the exciting line. The exciting radiation is a line and is directed onto the sample at an angle larger than the critical angle between the substrate (16) and the sample (14) so that the exciting radiation undergoes total internal reflection at the substrate-sample boundary and is damped. The beam shaping element (18) is designed to shape the beam into a an annular shape (34), and the line forming element (20) is capable of shaping the annular beam, which is converted by the focusing device into an exciting line.

EFFECT: increasing the rate of measurement without losing sensitivity.

15 cl, 5 dwg