FIELD: chemistry.
SUBSTANCE: method of determining glutathione involves determination by cyclic voltammetry, wherein there is electrocatalytic oxidation of glutathione on a graphite electrode modified with silver particles. The method according to the invention involves modifying graphite electrodes with colloidal silver particles from a silver sol for 300 s at electrolysis potential of -1.0 V, followed by recording anodic maxima of electrooxidation of glutathione on an anodic curve with potential sweep rate of 100 mV/s on a background of 0.1 M NaOH solution in the potential range from -1.0 V to 1.0 V; concentration of glutathione is determined from the height of anodic maxima of current versus voltage curves in the potential range from 0.30 V to 0.60 V relative a saturated silver chloride electrode by standard addition method.
EFFECT: high sensitivity of the method of determining glutathione.
2 ex, 1 tbl, 2 dwg
Authors
Dates
2014-03-20—Published
2012-11-06—Filed