FIELD: medicine.
SUBSTANCE: cell substrate is stained simultaneously with anti-CD34 monoclonal antibodies and nucleotropic (nuclear) strain Syto16, and the precursor cells (CD34+) are counted within the Syto16+ cells.
EFFECT: using the given method enables counting the quantity of the whole population of CD34+CD45 cells within the nuclear Syto16+ cells with a domain of analysis unlimited on the basis of anti-CD45 expression, and avoiding a lower percentage of the CD34+ precursor cells.
1 ex, 2 dwg
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