FIELD: biotechnology.
SUBSTANCE: for bioremediation of cadmium-contaminated soils first before sowing peas, the inoculum is prepared from symbiotic microorganisms resistant to cadmium, consisting of soil-root mixture containing the strain of arbuscular mycorrhiza fungus Glomus sp.Fo 1 deposited in the collection of state scientific institution All-Russian Research Institute for Agricultural Microbiology of Russian Agricultural Academy under the number RCAM00630, in the amount of 0.5-1.0 g of a mixture per 1 pea seed; the aqueous suspension of nodule bacteria containing the strain Rhizobium leguminosarum bv. viciae deposited in the collection of state scientific institution All-Russian Research Institute for Agricultural Microbiology of Russian Agricultural Academy under the number RCAM1066, containing 106-107 cells of bacteria per 1 ml of the suspension, in the amount of 0.1-0.5 ml suspension per 1 pea seed, and the aqueous suspension of associative bacteria containing the strain Variovorax paradoxus 5C-2, deposited in the collection of state scientific institution All-Russian Research Institute for Agricultural Microbiology of Russian Agricultural Academy under the number RCAM00049, containing 106-107 cells of bacteria per 1 ml of the suspension in an amount of 0.1-0.5 ml of the suspension per 1 pea seed. Then in the cadmium-contaminated soil the seeds of genetically modified peas SGECdt are sown, which have higher resistance and accumulation of cadmium in an amount of 20-60 seeds per 1 m2, combining them during sowing with an inoculum following the specified standards, and the plants are grown to full maturity of beans, then the peas are mowed and removed from the soil surface.
EFFECT: accelerated recovery process of healthy biocenosis of soil.
2 tbl
