FIELD: medicine.
SUBSTANCE: invention relates to the field of sterilisation and can be used in control of sterilisation of materials and products, for instance, for medical or veterinary purposes. A method of controlling sterilisation of materials and products includes placement of biological indicators with spores of cells of test-organisms among sterilised objects before sterilisation, with immediate introduction of (0.3÷0.5) cm3 of a chemiluminiscent composition into the biological indicators with the spores of cells of the test microorganisms after the end of sterilisation, the spores of cells of the test-microorganisms are lysed by shaking indicators for (3÷7) min, after which the presence of viable cells of the test-organisms in the obtained extract is determined by a chemiluminiscent method and efficiency of sterilisation is determined by an intensity of cheluminiscence. The absence of chemiluminiscence in the analysis of each of the obtained extracts is an indicator of efficient sterilisation of materials and products.
EFFECT: invention ensures reduction of time of control of the sterilisation process efficiency to 25÷30 min with simultaneous simplification of the process technology.
6 cl, 8 dwg, 4 tbl, 4 ex
