FIELD: biotechnology; fishing and fish farming.
SUBSTANCE: disclosed is a group of inventions related to biotechnology and fish farming: a combined probiotic preparation for use in aquaculture cultivation, which includes bacterial strains Bacillus subtilis VKM B-3154D, Bacillus subtilis VKM B-3171D, Bacillus licheniformis VKM-B-3172D, adapted to growth in conditions of low temperatures (temperatures of aquaculture habitation) and at the same time having very high thermal stability; method of producing a combined probiotic preparation. Proposed preparation contains a mixture of biomass of two Bacillus subtilis strains and Bacillus licheniformis strain in spore form and maltodextrin excipient. Method of preparing the preparation involves separate cultivation of said strains on a liquid nutrient medium, concentration of cultural liquids, drying of concentrates and their mixing, introduction of maltodextrin excipient into a mixture of dry concentrates with obtaining in the finished preparation not less than 5×109 CFU/g of viable bacteria.
EFFECT: group of inventions provides a preparation based on strains capable of growth in a wide temperature range in a spore form, which retain viability in unfavorable environmental conditions, including at high temperatures, which is important when producing the preparation and ensuring its long storage life; use of the new combined probiotic increases efficiency of prevention and treatment of aquaculture and feed conversion.
6 cl, 5 tbl, 6 ex
