SINGLE-STRANDED CIRCULAR RNA AND METHOD FOR PRODUCING IT Russian patent published in 2014 - IPC C12N15/11 C12N15/10 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biochemistry, particularly to a method for producing a single-stranded circular RNA. The method involves a synthesis of a sense chain and an anti-sense chain containing a nucleotide sequence with unpaired nucleotides on 5'-terminal and 3'-terminal, in a combination with a ligation of the nucleotide on 5'-terminal of the nucleotide sequence with the unpaired nucleotides in the sense chain with the nucleotide on 3'-terminal of the nucleotide sequence with the unpaired nucleotides in the anti-sense chain and vice versa with using lygase. The nucleotide sequence with the unpaired nucleotides on 5'-terminal of the sense chain and the nucleotide sequence with the unpaired nucleotides on 3'-terminal of the anti-sense chain are looped together. The nucleotide sequence with the unpaired nucleotides on 3'-terminal of the sense chain and the nucleotide sequence with the unpaired nucleotides on 5'-terminal of the anti-sense chain are also looped together. The sense and anti-sense chains are stemmed together. The loop length makes 9 nucleotides, while the stem length makes 19 to 31 nucleotides.

EFFECT: producing the single-stranded circular RNA.

3 cl, 9 dwg, 5 ex