DEVICE, CONTAINING CARDIOMYOCYTES, AND METHOD OF PRODUCTION AND APPLICATION THEREOF Russian patent published in 2014 - IPC C12M3/04 C12N5/00 

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions relates to the field of medications and control of their effect. A device for the determination of a chemical compound cardiotoxicity contains a substrate (10), carrying a deformable unit (34), with the unit (34) being partially separated from the substrate by a cavity (32), providing the extraplanar unit deformation. The unit (34) contains the first deformable layer (16), the second deformable layer (20) and a multi-electrode structure (18), layered between the first and second deformable layers. The second deformable layer contains glued to it a cardiomyocyte template (28) and a container for liquid (26). The latter is installed on the substrate for producing an impact on cardiomyocytes with the chemical compound. A method of the claimed device manufacturing includes growth of an oxide layer (12) on the substrate (10), application of the first deformable layer (16) on the oxide layer, application and templating a conductive layer (18) on the first deformable layer with the formation of a multi-electrode structure, application of the second deformable layer (20) on the first deformable layer, templating the second deformable layer to provide access to the multi-electrode structure, application of an adhesive template (24) on the templated second deformable layer, gluing cardiomyocytes (28) to the adhesive template and formation of the cavity (32) under the first deformable layer. The application of the device for purposive search of medications and/or elaboration of medications and to the method of elaboration of a model of a disease with respect to the disease, which is caused or modified with cell stretching, in particular the model of the cardiac disease.

EFFECT: group of inventions ensures an increased accuracy of determination of the chemical compound toxicity with simultaneous simplification of the construction and technology of application.

15 cl, 10 dwg