SUNFLOWER STERILITY/FERTILITY IDENTIFICATION METHOD Russian patent published in 2014 - IPC A01H1/04 

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly a method for molecular-genetic identification of sterility/fertility of sunflower pollen. The method includes analysis of the complete DNA of analysed samples for presence/absence of the mitochondrial gene orfH522 and marker sequence of the nuclear gene Rf1 via a multiplex polymerase chain reaction using a first pair of primers: agtagcccgttccgtgtttatgga and ctttctatttgggtcatcgccgga, which identifies the orfH522 gene of cytoplasmic male sterility of pollen (CMS PET1), and a second pair of primers: ggcatgatcaagtacataagcacagtc and tatgtacgggaatgagctccggtt, which identifies the marker sequence of the Rf1 gene - fertility restorer of pollen CMS PET1, wherein a sample is defined as fertile if a) both orfH522 and the Rf1 gene marker are present, b) orfH522 is absent and the Rf1 gene marker are present, c) both orfH522 and the Rf1 gene marker are absent, and a sample is defined as sterile if orfH522 is present and the Rf1 gene marker is absent. Disclosed is a diagnostic set, which includes primers, for molecular-genetic identification of sterility/fertility of sunflower pollen using said method, as well as use of said method in plant selection.

EFFECT: invention provides effective determination of sterility/fertility of sunflower pollen.

7 cl, 6 dwg, 1 tbl