SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBE FOR SPECIES-SPECIFIC EXPRESS-IDENTIFICATION OF RNA OF MACHUPO VIRUS BY METHOD OF POLYMERASE CHAIN REACTION IN REAL TIME Russian patent published in 2014 - IPC C12Q1/68 C12N7/00 

Abstract RU 2525937 C1

FIELD: chemistry.

SUBSTANCE: invention deals with a set of oligonucleotide primers and a fluorescently labelled probe for species-specific express-identification of RNA of the Machupo virus by a method of polymerase chain reaction in real time. The claimed set includes sequences of oligonucleotides, species-specific for the Machupo virus and having the following structure: external: 5'→3' 5' TCCTCYTCACCCCTTTTGTTCTTTCT 3' internal: 5'→3' 5' CGCACAGTGGATCCTAGGCA 3' probe: R6G - TGAGTCCACCGRAAGCTGGGRATYTCYTT - BHQ1.

EFFECT: possibility of the application in medical practice for determination of a genetic material of the Machupo virus in clinical or sectional samples.

2 dwg, 1 tbl, 2 ex

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RU 2 525 937 C1

Authors

Ternovoj Vladimir Aleksandrovich

Shikov Andrej Nikolaevich

Sementsova Aleksandra Olegovna

Agafonov Aleksandr Petrovich

Sergeev Aleksandr Nikolaevich

Dates

2014-08-20Published

2013-07-30Filed