FIELD: chemistry.
SUBSTANCE: invention deals with a set of oligonucleotide primers and a fluorescently labelled probe for species-specific express-identification of RNA of the Machupo virus by a method of polymerase chain reaction in real time. The claimed set includes sequences of oligonucleotides, species-specific for the Machupo virus and having the following structure: external: 5'→3' 5' TCCTCYTCACCCCTTTTGTTCTTTCT 3' internal: 5'→3' 5' CGCACAGTGGATCCTAGGCA 3' probe: R6G - TGAGTCCACCGRAAGCTGGGRATYTCYTT - BHQ1.
EFFECT: possibility of the application in medical practice for determination of a genetic material of the Machupo virus in clinical or sectional samples.
2 dwg, 1 tbl, 2 ex
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Authors
Dates
2014-08-20—Published
2013-07-30—Filed