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2 487 942

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IPC

C12P19/30(2006-01-01)

C12Q1/68(2006-01-01)

C12N7/00(2006-01-01)

(21) (22)

Application

2012104464/10, 2012-02-08

(24)

Start date

2011-07-19

(22)

Actual filing date

2012-02-08

(45)

Published

2013-07-20

(72)

Inventor

Shikov Andrej NikolaevichTernovoj Vladimir AleksandrovichSementsova Aleksandra OlegovnaAgafonov Aleksandr PetrovichSergeev Aleksandr Nikolaevich

(73)

Holder

Federal'Noe Bjudzhetnoe Uchrezhdenie Nauki Nauchnyj Tsentr Virusologii I Biotekhnologii Gnts Vb

SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBES FOR TYPE-SPECIFIC EXPRESS IDENTIFICATION OF EBOLA-ZAIRE VIRUS BY METHOD OF POLYMERASE CHAIN REACTION Russian patent published in 2013 - IPC C12P19/30 C12Q1/68 C12N7/00

Abstract RU 2487942 C1

FIELD: biotechnologies.

SUBSTANCE: invention relates to a set of oligonucleotide primers and fluorescent-marked probes for type-specific express-identification of the Ebola-Zaire virus by the method of polymerase chain reaction in real time. The set includes sequences that are type-specific for the Ebola-Zaire virus: external: 5'→' 5' CCACTTTTCTCAACCAAAATTATTAGTGA 3' 3'←5' 5'TTCTCTAAATCAGTTACAAARCTACTCCC 3' internal: 5'→3' 5'TGGGATCCAGTHTIYGARCC 3' 3'←5' 5' ACTACCATCATATTGCTAGGAAATGCTT 3' probe: FAM - TACTACCACAATATCGGAACTTTTCTTTCTCATTGAA - BHQ1.

EFFECT: invention may be used in medicine for quick detection of genetic material of Ebola-Zaire virus.

1 dwg, 3 tbl, 2 ex

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RU 2 487 942 C1

Authors

Shikov Andrej Nikolaevich

Ternovoj Vladimir Aleksandrovich

Sementsova Aleksandra Olegovna

Agafonov Aleksandr Petrovich

Sergeev Aleksandr Nikolaevich

Dates

2013-07-20—Published

2012-02-08—Filed