FIELD: biotechnologies.
SUBSTANCE: invention relates to a set of oligonucleotide primers and fluorescent-marked probes for type-specific express-identification of the Ebola-Zaire virus by the method of polymerase chain reaction in real time. The set includes sequences that are type-specific for the Ebola-Zaire virus: external: 5'→' 5' CCACTTTTCTCAACCAAAATTATTAGTGA 3' 3'←5' 5'TTCTCTAAATCAGTTACAAARCTACTCCC 3' internal: 5'→3' 5'TGGGATCCAGTHTIYGARCC 3' 3'←5' 5' ACTACCATCATATTGCTAGGAAATGCTT 3' probe: FAM - TACTACCACAATATCGGAACTTTTCTTTCTCATTGAA - BHQ1.
EFFECT: invention may be used in medicine for quick detection of genetic material of Ebola-Zaire virus.
1 dwg, 3 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELED PROBES FOR SPECIES-SPECIFIC EXPRESS-IDENTIFICATION OF VIRUS EBOLA-SUDAN BY METHOD OF POLYMERASE CHAIN REACTION | 2012 |
|
RU2487167C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBES FOR SPECIES-SPECIFIC INSTANT IDENTIFICATION OF MARBURG VIRUS BY POLYMERASE CHAIN REACTION | 2011 |
|
RU2458143C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBE FOR SPECIES-SPECIFIC EXPRESS-IDENTIFICATION OF RNA OF MACHUPO VIRUS BY METHOD OF POLYMERASE CHAIN REACTION IN REAL TIME | 2013 |
|
RU2525937C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBE FOR SPECIES-SPECIFIC EXPRESS-IDENTIFICATION OF RNA OF JUNIN VIRUS BY METHOD OF POLYMERASE CHAIN REACTION IN REAL TIME | 2013 |
|
RU2525938C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBERS FOR SUBCOMPOUND-SPECIFIC IDENTIFICATION OF RNA OF DENGUE VIRUS BASED ON MULTIPLEX PCR IN REAL TIME | 2012 |
|
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| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR IDENTIFICATION OF TICK-BORNE ENCEPHALITIS VIRUS, WEST NILE FEVER, BORRELIA AND RICKETTSIA BY REAL TIME MULTIPLEX PCR | 2016 |
|
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| SET OF OLIGODESOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENCE MARKED PROBES FOR IDENTIFYING RNA OF HUMAN RESPIRATORY SYNCYTIAL VIRUS | 2013 |
|
RU2541773C2 |
| SET OF PRIMERS FOR IDENTIFICATION OF RNA STRAINS PUUMALA ORTHOHANTAVIRUS, COMMON IN THE REPUBLIC OF TATARSTAN | 2019 |
|
RU2720252C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBES FOR HUMAN RHINOVIRUS RNA IDENTIFICATION | 2013 |
|
RU2543151C2 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY MARKED PROBE IN TAQMAN FORMAT FOR DETECTION OF VIRUSES OF FLAVIVIRUS GENUS BY REAL-TIME PCR | 2022 |
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RU2808520C1 |
