FIELD: biotechnologies.
SUBSTANCE: invention relates to a set of oligonucleotide primers and fluorescent-marked probes for type-specific express-identification of the Ebola-Zaire virus by the method of polymerase chain reaction in real time. The set includes sequences that are type-specific for the Ebola-Zaire virus: external: 5'→' 5' CCACTTTTCTCAACCAAAATTATTAGTGA 3' 3'←5' 5'TTCTCTAAATCAGTTACAAARCTACTCCC 3' internal: 5'→3' 5'TGGGATCCAGTHTIYGARCC 3' 3'←5' 5' ACTACCATCATATTGCTAGGAAATGCTT 3' probe: FAM - TACTACCACAATATCGGAACTTTTCTTTCTCATTGAA - BHQ1.
EFFECT: invention may be used in medicine for quick detection of genetic material of Ebola-Zaire virus.
1 dwg, 3 tbl, 2 ex
Authors
Dates
2013-07-20—Published
2012-02-08—Filed