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2 487 167

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IPC

C12P19/30(2006-01-01)

C12Q1/68(2006-01-01)

C12N7/00(2006-01-01)

(21) (22)

Application

2012104465/10, 2012-02-08

(24)

Start date

2011-07-19

(22)

Actual filing date

2012-02-08

(45)

Published

2013-07-10

(72)

Inventor

Shikov Andrej NikolaevichTernovoj Vladimir AleksandrovichSementsova Aleksandra OlegovnaAgafonov Aleksandr PetrovichSergeev Aleksandr Nikolaevich

(73)

Holder

Federal'Noe Bjudzhetnoe Uchrezhdenie Nauki Nauchnyj Tsentr Virusologii I Biotekhnologii Gnts Vb

SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELED PROBES FOR SPECIES-SPECIFIC EXPRESS-IDENTIFICATION OF VIRUS EBOLA-SUDAN BY METHOD OF POLYMERASE CHAIN REACTION Russian patent published in 2013 - IPC C12P19/30 C12Q1/68 C12N7/00

Abstract RU 2487167 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology and is related to the set oligonucleotide primers and fluorescent-labeled probes for species-specific express-identification the virus Ebola-Sudan by the method of polymerase chain reaction in real time. The set comprises sequences species-specific for the virus Ebola-Sudan: external: 5'→3' 5' CCGTTATTCTCYACRAAGRTSATTAGTGA 3' 3'←5' 5' TTCTCTAGGTCTGTGACAAAACTACTCCC 3' internal: 5'→3' 5' TGGGATGCAGTHTTYGARCC 3' 3'←5' 5' ACAACCATCATRTTGCTTGGAAAGGCTT 3' probe: FAM - TATTGCCCCAGAATCGAAATTTTTCTTTTTCATTGAA-BHQ1.

EFFECT: invention can be used in medicine for rapid identification of the genetic material of the virus Ebola-Sudan.

1 dwg, 3 tbl, 2 ex

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RU 2 487 167 C1

Authors

Shikov Andrej Nikolaevich

Ternovoj Vladimir Aleksandrovich

Sementsova Aleksandra Olegovna

Agafonov Aleksandr Petrovich

Sergeev Aleksandr Nikolaevich

Dates

2013-07-10—Published

2012-02-08—Filed