RECOMBINANT PLASMID DNA pPA-OPRF-ETA CODING SYNTHESIS OF RECOMBINANT PROTEIN OPRF-ETA Pseudomonas aeruginosa, STRAIN Escherichia coli PA-OPRF-ETA PRODUCING RECOMBINANT PROTEIN OPRF-ETA Pseudomonas aeruginosa AND METHOD FOR PREPARING RECOMBINANT PROTEIN OPRF-ETA Pseudomonas aeruginosa Russian patent published in 2014 - IPC C12N1/21 C12N15/09 C12N15/31 C12P21/00 C12R1/19 

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions refers to biotechnology. The recombinant plasmid DNA pPA-OPRF-ETA having a size of 8007 base pairs codes the synthesis of recombinant protein OprF-ETA Pseudomonas aeruginosa. The plasmid contains the following fragments: Xba I-Hind III DNA fragment having a size 177 base pairs; Hind III-Hind III DNA fragment having a size of 1059 base pairs; Hind III-Xho I DNA fragment having a size of 1598 base pairs; Sph I-Hind III DNA fragment having a size of 425 base pairs; EcoR I - BamH I DNA fragment having a size of 57 base pairs. There are also presented the bacterial strain Escherichia coli PA-OPRF-ETA producing protein OprF-ETA and prepared by transforming the parent bacterial strain Escherichia coli BL21(DE3) of plasmid DNA pPA-OPRF-ETA and a method for preparing the above protein OprF-ETA.

EFFECT: group of inventions enables preparing the target product weighing approximately 100 kDa.

3 cl, 3 dwg, 1 tbl, 4 ex