FIELD: biotechnology, microbiology.
SUBSTANCE: invention relates to a method for preparing bacterium pyocyanic anatoxin used in specific therapy and prophylaxis of infections caused by Pseudomonas aeruginosa (pyocyanic bacterium). Method involves culturing microorganisms in liquid nutrient medium comprising the following components per 1 liter of distilled water: citric acid, 4.9-5.0; sodium chloride, 0.9-1.0; magnesium sulfate, 4.9-5.0; ferrous sulfate, 0.04-0.05; asparagines, 0.9-1.0; glucose, 0.9-1.0; glycerol, 2.9-3.0 ml, balance, to the final concentration of pyocyanic bacterium, 14-15 billion of microbial bodies in 1 ml. Then the prepared biomass-containing cultural fluid is subjected for sterilization by autoclaving at 0.6-0.7 atm for 45-50 min followed by detoxification with formalin and anatoxin-containing cultural fluid is separated from biomass by filtration and sorbed its on aluminum hydroxide gel. Using the invention provides enhancing antigenic activity of toxic product and to standardize condition for culturing the producer.
EFFECT: improved preparing method.
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