RECOMBINANT PLASMID pPA-OPRFI DNA CODING HYBRID RECOMBINANT F-I PROTEIN OF Pseudomonas aeruginosa OUTER MEMBRANE, STRAIN Escherichia coli PA-OPRFI PRODUCING HYBRID RECOMBINANT F-I PROTEIN OF Pseudomonas aeruginosa OUTER MEMBRANE AND METHOD FOR PRODUCING HYBRID RECOMBINANT F-I PROTEIN OF Pseudomonas aeruginosa OUTER MEMBRANE Russian patent published in 2014 - IPC C12N15/09 C12N15/31 C12N15/62 C12N1/21 C07K1/00 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology and concerns recombinant plasmid pPA-OPRFI DNA coding hybrid recombinant F-I protein of Pseudomonas aeruginosa outer membrane, of the bacterial strain E.coli PA-OPRFI producing this hybrid protein, and a method for producing this recombinant protein. The presented plasmid DNA contains a DNA fragment containing a sequence of modified promoter of bacteriophage T5 and two lactose operons; a DNA fragment containing a ribosome entry site, an initiation ATG-codon and a sequence coding six histidines; a DNA fragment containing the full-size sequences of oprF and opri genes of P.aeruginosa; a DNA fragment containing a ribosome entry site, and a DNA fragment containing a lambda t₀ transcription stop region.

EFFECT: presented inventions enables producing the hybrid recombinant F-I protein of Paeruginosa outer membrane for carrying out an immunobiological assay in developing a Pseudomonas aeruginosa vaccine, and also for producing donor immunoglobulins for therapy of active forms of Paeruginosa infection.

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